PDBe 3e22

X-ray diffraction
3.8Å resolution

Tubulin-colchicine-soblidotin: Stathmin-like domain complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1C chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 449 amino acids
Theoretical weight: 50.09 KDa
Source organism: Bos taurus
UniProt:
  • Best match: Q3ZCJ7 (Residues: 1-449)
Gene name: TUBA1C
Tubulin beta-2B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 445 amino acids
Theoretical weight: 49.97 KDa
Source organism: Bos taurus
UniProt:
  • Best match: Q6B856 (Residues: 1-445)
Gene name: TUBB2B
Stathmin-4 Chain: E
Molecule details ›
Chain: E
Length: 142 amino acids
Theoretical weight: 16.71 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Best match: P63043 (Residues: 49-189)
Gene name: Stmn4

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P65
Unit cell:
a: 324.093Å b: 324.093Å c: 53.28Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.231 0.229 0.295
Expression system: Escherichia coli