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Crystal structure of EcTrmB in complex with SAM

X-ray diffraction
1.5Å resolution
PDB entry 3dxy coloured by chain, front view.
PDB entry 3dxy coloured by chain, side view.
PDB entry 3dxy coloured by chain, top view.
Source organism: Escherichia coli K-12
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: tRNA (guanine-N(7)-)-methyltransferase
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Multimeric state: monomeric
Accessible surface area: 10596.43 Å2
Buried surface area: 817.27 Å2
Dissociation area: 69.17 Å2
Dissociation energy (ΔGdiss): -0.35 kcal/mol
Dissociation entropy (TΔSdiss): 0.88 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-141703

Macromolecules

tRNA (guanine-N(7)-)-methyltransferase
Chain: A
Length: 218 amino acids
Theoretical weight: 24.83 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A8I5 (Residues: 33-239; Coverage: 87%)
Gene names: JW2927, b2960, trmB, trmI, yggH
Pfam: Putative methyltransferase
InterPro:
CATH: Vaccinia Virus protein VP39
tRNA (guanine-N(7)-)-methyltransferase in PDB entry 3dxy, assembly 1, front view.
tRNA (guanine-N(7)-)-methyltransferase in PDB entry 3dxy, assembly 1, side view.
tRNA (guanine-N(7)-)-methyltransferase in PDB entry 3dxy, assembly 1, top view.
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