X-ray diffraction
1.5Å resolution

Crystal structure of EcTrmB in complex with SAM


Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
tRNA (guanine-N(7)-)-methyltransferase Chain: A
Molecule details ›
Chain: A
Length: 218 amino acids
Theoretical weight: 24.83 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P0A8I5 (Residues: 33-239; Coverage: 87%)
Gene names: JW2927, b2960, trmB, trmI, yggH
Sequence domains: Putative methyltransferase
Structure domains: Vaccinia Virus protein VP39

Ligands and Environments

Cofactor: Ligand SAM 1 x SAM
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 3W1A
Spacegroup: P21
Unit cell:
a: 46.04Å b: 46.76Å c: 58.13Å
α: 90° β: 90.4° γ: 90°
R R work R free
0.155 0.154 0.185
Expression system: Escherichia coli