3dxa

X-ray diffraction
3.5Å resolution

Crystal Structure of the DM1 TCR in complex with HLA-B*4405 and decamer EBV antigen

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
HLA class I histocompatibility antigen, B alpha chain Chains: A, F, K
Molecule details ›
Chains: A, F, K
Length: 276 amino acids
Theoretical weight: 32.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01889 (Residues: 25-300; Coverage: 82%)
Gene names: HLA-B, HLAB
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, G, L
Molecule details ›
Chains: B, G, L
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Epstein-Barr nuclear antigen 6 Chains: C, H, M
Molecule details ›
Chains: C, H, M
Length: 10 amino acids
Theoretical weight: 1.28 KDa
Source organism: Human herpesvirus 4 strain B95-8
Expression system: Not provided
UniProt:
  • Canonical: P03204 (Residues: 281-290; Coverage: 1%)
Gene names: BERF3-BERF4, EBNA6
T cell receptor alpha constant Chains: D, I, N
Molecule details ›
Chains: D, I, N
Length: 199 amino acids
Theoretical weight: 22.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01848 (Residues: 1-91; Coverage: 65%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
DM1 T cell receptor beta chain Chains: E, J, O
Molecule details ›
Chains: E, J, O
Length: 244 amino acids
Theoretical weight: 28.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P6522
Unit cell:
a: 121.954Å b: 121.954Å c: 695.914Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.289 0.286 0.33
Expression systems:
  • Escherichia coli
  • Not provided