PDBe 3dtp

Electron Microscopy
20Å resolution

Tarantula heavy meromyosin obtained by flexible docking to Tarantula muscle thick filament Cryo-EM 3D-MAP

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero 216-mer (preferred)
hetero hexamer
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Myosin II regulatory light chain Chains: E, F
Molecule details ›
Chains: E, F
Length: 196 amino acids
Theoretical weight: 21.71 KDa
Source organism: Avicularia avicularia
UniProt:
  • Canonical: B4XT43 (Residues: 1-196; Coverage: 100%)
Gene name: RLC
Sequence domains:
Myosin light polypeptide 6 Chains: C, D
Molecule details ›
Chains: C, D
Length: 150 amino acids
Theoretical weight: 16.87 KDa
Source organism: Gallus gallus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P02607 (Residues: 2-151; Coverage: 99%)
Gene name: MYL6
Myosin-11; Myosin-7 Chain: A
Molecule details ›
Chain: A
Length: 971 amino acids
Theoretical weight: 111.85 KDa
Source organisms: Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P10587 (Residues: 2-852; Coverage: 43%)
  • Canonical: P12883 (Residues: 842-961; Coverage: 6%)
  • nullnull
Gene names: MYH11, MYH7, MYHCB
Sequence domains:
Myosin-7; Myosin-11 Chain: B
Molecule details ›
Chain: B
Length: 973 amino acids
Theoretical weight: 112.05 KDa
Source organisms: Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P10587 (Residues: 2-852; Coverage: 43%)
  • Canonical: P12883 (Residues: 842-963; Coverage: 6%)
  • nullnull
Gene names: MYH11, MYH7, MYHCB
Sequence domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 20Å
Relevant EMDB volumes: EMD-1950
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21