X-ray diffraction
1.6Å resolution

HIV-1 capsid C-terminal domain mutant (E187A) in complex with an inhibitor of particle assembly (CAI)


Function and Biology Details

Reactions catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Specific for a P1 residue that is hydrophobic, and P1' variable, but often Pro.
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Capsid protein p24 Chain: A
Molecule details ›
Chain: A
Length: 86 amino acids
Theoretical weight: 9.47 KDa
Source organism: Human immunodeficiency virus type 1 (NEW YORK-5 ISOLATE)
Expression system: Escherichia coli
  • Canonical: P12497 (Residues: 278-363; Coverage: 6%)
Gene name: gag-pol
Structure domains: Non-ribosomal Peptide Synthetase Peptidyl Carrier Protein; Chain A
Peptide Inhibitor of capsid assembly Chain: T
Molecule details ›
Chain: T
Length: 12 amino acids
Theoretical weight: 1.45 KDa
Source organism: Human immunodeficiency virus type 1 (NEW YORK-5 ISOLATE)
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 42.894Å b: 42.894Å c: 90.813Å
α: 90° β: 90° γ: 90°
R R work R free
0.215 0.212 0.272
Expression systems:
  • Escherichia coli
  • Not provided