› 3drw

X-ray diffraction
1.9Å resolution

Crystal Structure of a Phosphofructokinase from Pyrococcus horikoshii OT3 with AMP

Released:
DOI: 10.2210/pdb3drw/pdb
PDB entry 3drw coloured by chain, front view.
PDB entry 3drw coloured by chain, side view.
PDB entry 3drw coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
ADP-dependent 6-phosphofructokinase from Pyrococcus horikoshii OT3: structure determination and biochemical characterization of PH1645.
Currie MA, Merino F, Skarina T, Wong AH, Singer A, Brown G, Savchenko A, Caniuguir A, Guixé V, Yakunin AF, Jia Z
J Biol Chem 284 22664-71 (2009)
PMID: 19553681

Function and Biology Details

Reaction catalysed: 
ADP + D-fructose 6-phosphate = AMP + D-fructose 1,6-bisphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129852 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ADP-specific phosphofructokinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 474 amino acids
Theoretical weight: 54.5 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O59355 (Residues: 1-450; Coverage: 100%)
Gene names: PH1645, pfkC
Sequence domains: ADP-specific Phosphofructokinase/Glucokinase conserved region
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand AMP 2 x AMP
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 68.464Å b: 104.19Å c: 70.844Å
α: 90° β: 105.09° γ: 90°
R-values:
R R work R free
0.174 0.172 0.224
Expression system: Escherichia coli

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Citations

1 review citation

Carbohydrate metabolism in Archaea: current insights into unusual enzymes and pathways and their regulation.
Bräsen et al. (2014)
5 other articles

5 mentions without citation

ADP-dependent 6-phosphofructokinase from Pyrococcus horikoshii OT3: structure determination and biochemical characterization of PH1645.
Currie et al. (2009)
4 more