3dr3

X-ray diffraction
2Å resolution

Structure of IDP00107, a potential N-acetyl-gamma-glutamylphosphate reductase from Shigella flexneri

Released:
Source organism: Shigella flexneri
Entry authors: Singer AU, Skarina T, Onopriyenko O, Edwards AM, Anderson WF, Savchenko A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
N-acetyl-L-glutamate 5-semialdehyde + NADP(+) + phosphate = N-acetyl-L-glutamyl 5-phosphate + NADPH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
N-acetyl-gamma-glutamyl-phosphate reductase Chain: A
Molecule details ›
Chain: A
Length: 337 amino acids
Theoretical weight: 36.24 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli
UniProt:
  • Canonical: P59310 (Residues: 1-334; Coverage: 100%)
Gene names: S3711, SF4035, argC
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P6222
Unit cell:
a: 117.299Å b: 117.299Å c: 115.472Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.168 0.166 0.216
Expression system: Escherichia coli