PDBe 3dqk

X-ray diffraction
1.4Å resolution

Structure of the Yellow Fluorescent Protein Citrine Frozen at 1000 Atmospheres Number 2: Structure 6 in a Series of 26 High Pressure Structures

Released:
Source organism: Aequorea victoria
Primary publication:
Alteration of citrine structure by hydrostatic pressure explains the accompanying spectral shift.
Proc. Natl. Acad. Sci. U.S.A. 105 13362-6 (2008)
PMID: 18768811

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 241 amino acids
Theoretical weight: 27.39 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 51.362Å b: 62.928Å c: 71.297Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.265
Expression system: Escherichia coli BL21(DE3)