Function and Biology Details
Reactions catalysed:
3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid
Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Assembly name:
Pol protein (preferred)
PDBe Complex ID:
PDB-CPX-108510 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Pol protein
Molecule details ›
Chain: A
Length: 560 amino acids
Theoretical weight: 64.59 KDa
Source organism: HIV-1 M:B_HXB2R
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 560 amino acids
Theoretical weight: 64.59 KDa
Source organism: HIV-1 M:B_HXB2R
Expression system: Escherichia coli
UniProt:
- Canonical:
A7YKL0 (Residues: 161-720; Coverage: 56%)
Sequence domains:
- Reverse transcriptase (RNA-dependent DNA polymerase)
- Reverse transcriptase thumb domain
- Reverse transcriptase connection domain
- RNase H
Pol protein
Molecule details ›
Chain: B
Length: 440 amino acids
Theoretical weight: 51.4 KDa
Source organism: HIV-1 M:B_HXB2R
Expression system: Escherichia coli
UniProt:
Sequence domains:
Length: 440 amino acids
Theoretical weight: 51.4 KDa
Source organism: HIV-1 M:B_HXB2R
Expression system: Escherichia coli
UniProt:
- Canonical: A7YKL0 (Residues: 161-600; Coverage: 44%)
Sequence domains:
- Reverse transcriptase (RNA-dependent DNA polymerase)
- Reverse transcriptase thumb domain
- Reverse transcriptase connection domain
