3dmp

X-ray diffraction
2.6Å resolution

2.6 A crystal structure of uracil phosphoribosyltransferase from Burkholderia pseudomallei

Released:
Source organism: Burkholderia pseudomallei
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed:
UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uracil phosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 217 amino acids
Theoretical weight: 24.2 KDa
Source organism: Burkholderia pseudomallei
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q63VS8 (Residues: 1-216; Coverage: 100%)
Gene names: BPSL1166, upp
Structure domains: Rossmann fold

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: C2
Unit cell:
a: 102.222Å b: 80.637Å c: 141.833Å
α: 90° β: 99.11° γ: 90°
R-values:
R R work R free
0.221 0.219 0.271
Expression system: Escherichia coli BL21(DE3)