› 3dlh

X-ray diffraction
3Å resolution

Crystal structure of the guide-strand-containing Argonaute protein silencing complex

Released:
DOI: 10.2210/pdb3dlh/pdb
PDB 3dlh coloured by chain and viewed from the front.
PDB 3dlh coloured by chain and viewed from the side.
PDB 3dlh coloured by chain and viewed from the top.
Source organism: Thermus thermophilus HB27
Primary publication:
Structure of the guide-strand-containing argonaute silencing complex.
Wang Y, Sheng G, Juranek S, Tuschl T, Patel DJ
Nature 456 209-13 (2008)
PMID: 18754009

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Piwi domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 685 amino acids
Theoretical weight: 76.73 KDa
Source organism: Thermus thermophilus HB27
Expression system: Escherichia coli
UniProt:
  • Canonical: Q746M7 (Residues: 1-685; Coverage: 100%)
Gene name: TT_P0026
Sequence domains:
Structure domains:
DNA (5'-D(DTP*DGP*DAP*DGP*DGP*DTP*DAP*DGP*DTP*DAP*DGP*DGP*DTP*DTP*DGP*DTP*DAP*DTP*DAP*DGP*DT)-3') Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 21 nucleotides
Theoretical weight: 6.59 KDa
Source organism: Thermus thermophilus HB27
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand MG 3 x MG
Ligand PGE 1 x PGE
Ligand PO4 2 x PO4
Ligand ACY 2 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P212121
Unit cell:
a: 74.744Å b: 114.629Å c: 175.689Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.284
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

65 review citations

Exosomal MicroRNAs and Organotropism in Breast Cancer Metastasis.
Wong et al. (2020)
64 more

24 mentions without citation

DNA interference and beyond: structure and functions of prokaryotic Argonaute proteins.
Lisitskaya et al. (2018)
23 more