› › Function and Biology

Complex of elongating Escherichia coli 70S ribosome and EF4(LepA)-GMPPNP

Source organism: Escherichia coli K-12
Biochemical function: protein binding
Biological process: translation
Cellular component: cytosol

Quick links

EC 3.6.5.n1: Elongation factor 4

Reaction catalysed:
GTP + H(2)O = GDP + phosphate
Systematic name:
GTP phosphohydrolase (protein-synthesis-assisting, ribosomal back translocating)
Alternative Name(s):
  • Ribosomal back translocase
  • Ribosomal back-translocase LepA

GO terms

Biochemical function:
Biological process:
Cellular component:

Sequence families

Pfam Protein families (Pfam)
PF00298
Domain description: Ribosomal protein L11, RNA binding domain
Occurring in:
  1. Large ribosomal subunit protein uL11
The deposited structure of PDB entry 3deg contains 1 copy of Pfam domain PF00298 (Ribosomal protein L11, RNA binding domain) in Large ribosomal subunit protein uL11. Showing 1 copy in chain K [auth H].

PF03946
Domain description: Ribosomal protein L11, N-terminal domain
Occurring in:
  1. Large ribosomal subunit protein uL11
The deposited structure of PDB entry 3deg contains 1 copy of Pfam domain PF03946 (Ribosomal protein L11, N-terminal domain) in Large ribosomal subunit protein uL11. Showing 1 copy in chain K [auth H].

PF00164
Domain description: Ribosomal protein S12/S23
Occurring in:
  1. Small ribosomal subunit protein uS12
The deposited structure of PDB entry 3deg contains 1 copy of Pfam domain PF00164 (Ribosomal protein S12/S23) in Small ribosomal subunit protein uS12. Showing 1 copy in chain J [auth D].

PF06421
Domain description: GTP-binding protein LepA C-terminus
Occurring in:
  1. Elongation factor 4
The deposited structure of PDB entry 3deg contains 1 copy of Pfam domain PF06421 (GTP-binding protein LepA C-terminus) in Elongation factor 4. Showing 1 copy in chain I [auth C].

PF00679
Domain description: Elongation factor G C-terminus
Occurring in:
  1. Elongation factor 4
The deposited structure of PDB entry 3deg contains 1 copy of Pfam domain PF00679 (Elongation factor G C-terminus) in Elongation factor 4. Showing 1 copy in chain I [auth C].

PF03144
Domain description: Elongation factor Tu domain 2
Occurring in:
  1. Elongation factor 4
The deposited structure of PDB entry 3deg contains 1 copy of Pfam domain PF03144 (Elongation factor Tu domain 2) in Elongation factor 4. Showing 1 copy in chain I [auth C].

PF00009
Domain description: Elongation factor Tu GTP binding domain
Occurring in:
  1. Elongation factor 4
The deposited structure of PDB entry 3deg contains 1 copy of Pfam domain PF00009 (Elongation factor Tu GTP binding domain) in Elongation factor 4. Showing 1 copy in chain I [auth C].

Rfam RNA families (Rfam)
RF00005
Domain description: tRNA
Occurring in:
  1. A/L-tRNA
  2. P-tRNA
The deposited structure of PDB entry 3deg contains 1 copy of Rfam domain RF00005 (tRNA) in A/L-tRNA. Showing 1 copy in chain A.
The deposited structure of PDB entry 3deg contains 1 copy of Rfam domain RF00005 (tRNA) in P-tRNA. Showing 1 copy in chain B.

RF02541
Domain description: Bacterial large subunit ribosomal RNA
Occurring in:
  1. 50S RNA helix 42-44
The deposited structure of PDB entry 3deg contains 1 copy of Rfam domain RF02541 (Bacterial large subunit ribosomal RNA) in 50S RNA helix 42-44. Showing 1 copy in chain E [auth G].

InterPro InterPro annotations
IPR005679
Domain description: Ribosomal protein uS12, bacterial-type
Occurring in:
  1. Small ribosomal subunit protein uS12
IPR000795
Domain description: Translational (tr)-type GTP-binding domain
Occurring in:
  1. Elongation factor 4
IPR000911
Domain description: Ribosomal protein uL11
Occurring in:
  1. Large ribosomal subunit protein uL11
IPR006032
Domain description: Small ribosomal subunit protein uS12
Occurring in:
  1. Small ribosomal subunit protein uS12
IPR006297
Domain description: Elongation factor 4
Occurring in:
  1. Elongation factor 4
IPR036769
Domain description: Large ribosomal subunit protein uL11, C-terminal domain superfamily
Occurring in:
  1. Large ribosomal subunit protein uL11
IPR036796
Domain description: Large ribosomal subunit protein uL11, N-terminal domain superfamily
Occurring in:
  1. Large ribosomal subunit protein uL11
IPR012340
Domain description: Nucleic acid-binding, OB-fold
Occurring in:
  1. Small ribosomal subunit protein uS12
IPR035647
Domain description: EF-G domain III/V-like
Occurring in:
  1. Elongation factor 4
IPR027417
Domain description: P-loop containing nucleoside triphosphate hydrolase
Occurring in:
  1. Elongation factor 4
IPR020785
Domain description: Large ribosomal subunit protein uL11, conserved site
Occurring in:
  1. Large ribosomal subunit protein uL11
IPR020783
Domain description: Large ribosomal subunit protein uL11, C-terminal
Occurring in:
  1. Large ribosomal subunit protein uL11
IPR020784
Domain description: Large ribosomal subunit protein uL11, N-terminal
Occurring in:
  1. Large ribosomal subunit protein uL11
IPR006519
Domain description: Large ribosomal subunit protein uL11, bacterial-type
Occurring in:
  1. Large ribosomal subunit protein uL11
IPR013842
Domain description: GTP-binding protein LepA, C-terminal
Occurring in:
  1. Elongation factor 4
IPR038363
Domain description: LepA, C-terminal domain superfamily
Occurring in:
  1. Elongation factor 4
IPR035654
Domain description: Elongation factor 4, domain IV
Occurring in:
  1. Elongation factor 4
IPR000640
Domain description: Elongation factor EFG, domain V-like
Occurring in:
  1. Elongation factor 4
IPR004161
Domain description: Translation elongation factor EFTu-like, domain 2
Occurring in:
  1. Elongation factor 4
IPR031157
Domain description: Tr-type G domain, conserved site
Occurring in:
  1. Elongation factor 4
IPR005225
Domain description: Small GTP-binding protein domain
Occurring in:
  1. Elongation factor 4

Structure domain

SCOP SCOP annotations
46907
Class: All alpha proteins
Fold: DNA/RNA-binding 3-helical bundle
Superfamily: Ribosomal protein L11, C-terminal domain
Occurring in:
  1. Large ribosomal subunit protein uL11
The deposited structure of PDB entry 3deg contains 1 copy of SCOP domain 46907 (Ribosomal protein L11, C-terminal domain) in Large ribosomal subunit protein uL11. Showing 1 copy in chain K [auth H].
54748
Class: Alpha and beta proteins (a+b)
Fold: Ribosomal L11/L12e N-terminal domain
Superfamily: Ribosomal L11/L12e N-terminal domain
Occurring in:
  1. Large ribosomal subunit protein uL11
The deposited structure of PDB entry 3deg contains 1 copy of SCOP domain 54748 (Ribosomal L11/L12e N-terminal domain) in Large ribosomal subunit protein uL11. Showing 1 copy in chain K [auth H].
50282
Class: All beta proteins
Fold: OB-fold
Superfamily: Nucleic acid-binding proteins
Occurring in:
  1. Small ribosomal subunit protein uS12
The deposited structure of PDB entry 3deg contains 1 copy of SCOP domain 50282 (Cold shock DNA-binding domain-like) in Small ribosomal subunit protein uS12. Showing 1 copy in chain J [auth D].

Quick links