3dc1

X-ray diffraction
2.5Å resolution

Crystal structure of kynurenine aminotransferase II complex with alpha-ketoglutarate

Released:

Function and Biology Details

Reactions catalysed:
L-2-aminoadipate + 2-oxoglutarate = 2-oxoadipate + L-glutamate
(1a) L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 425 amino acids
Theoretical weight: 47.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8N5Z0 (Residues: 1-425; Coverage: 100%)
Gene names: AADAT, KAT2, KYAT2
Structure domains:

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 60.93Å b: 72.09Å c: 109.079Å
α: 89.97° β: 100.65° γ: 93.88°
R-values:
R R work R free
0.217 0.215 0.244
Expression system: Escherichia coli BL21(DE3)