PDBe 3d9l

X-ray diffraction
2.2Å resolution

Snapshots of the RNA processing factor SCAF8 bound to different phosphorylated forms of the Carboxy-Terminal Domain of RNA-Polymerase II


Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
SR-related and CTD-associated factor 8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 16.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q9UPN6 (Residues: 1-136; Coverage: 11%)
Gene names: CCAP7, KIAA1116, RBM16, SCAF8
Sequence domains: RNA polymerase II-binding domain.
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
DNA-directed RNA polymerase II subunit RPB1 Chains: Y, Z
Molecule details ›
Chains: Y, Z
Length: 15 amino acids
Theoretical weight: 1.84 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P24928 (Residues: 1790-1803; Coverage: 1%)
Gene names: POLR2, POLR2A

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P43
Unit cell:
a: 56.296Å b: 56.296Å c: 106.739Å
α: 90° β: 90° γ: 90°
R R work R free
0.19 0.188 0.231
Expression systems:
  • Escherichia coli
  • Not provided