3d3f

X-ray diffraction
2.4Å resolution

Crystal Structure of Yvgn and cofactor NADPH from Bacillus subtilis

Released:
Source organism: Bacillus subtilis
Primary publication:
Structural and biochemical analyses of YvgN and YtbE from Bacillus subtilis.
Protein Sci. 18 1792-800 (2009)
PMID: 19585557

Function and Biology Details

Reaction catalysed:
(S)-lactaldehyde + NADP(+) = 2-oxopropanal + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glyoxal reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 275 amino acids
Theoretical weight: 31.57 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O32210 (Residues: 2-276; Coverage: 100%)
Gene names: BSU33400, yvgN
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments


Cofactor: Ligand NDP 2 x NDP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21212
Unit cell:
a: 80.517Å b: 123.285Å c: 57.226Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.236 0.306
Expression system: Escherichia coli