› 3cxn

X-ray diffraction
1.55Å resolution

Structure of the Urease Accessory Protein UreF from Helicobacter pylori

Released:
DOI: 10.2210/pdb3cxn/pdb
PDB entry 3cxn coloured by chain, front view.
PDB entry 3cxn coloured by chain, side view.
PDB entry 3cxn coloured by chain, top view.
Source organism: Helicobacter pylori
Primary publication:
Crystal structure of a truncated urease accessory protein UreF from Helicobacter pylori.
Lam R, Romanov V, Johns K, Battaile KP, Wu-Brown J, Guthrie JL, Hausinger RP, Pai EF, Chirgadze NY
Proteins 78 2839-48 (2010)
PMID: 20635345

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170760 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Urease accessory protein UreF Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 274 amino acids
Theoretical weight: 31.34 KDa
Source organism: Helicobacter pylori
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09065 (Residues: 2-254; Coverage: 100%)
Gene names: HP_0069, ureF
Sequence domains: UreF
Structure domains: Urease accessory protein UreF

Ligands and Environments

1 bound ligand:
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 33 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: C2
Unit cell:
a: 135.225Å b: 89.418Å c: 66.042Å
α: 90° β: 94.03° γ: 90°
R-values:
R R work R free
0.188 0.187 0.211
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Functional and structural aspects of Helicobacter pylori acidic stress response factors.
Zanotti et al. (2010)
2 more

3 mentions without citation

Biosynthesis of the urease metallocenter.
Farrugia et al. (2013)
2 more