3cx4

X-ray diffraction
2.29Å resolution

Crystal Structure of E.coli GS mutant E377A in complex with ADP and oligosaccharides

Released:
Source organism: Escherichia coli
Primary publication:
Oligosaccharide binding in Escherichia coli glycogen synthase.
Biochemistry 48 10089-97 (2009)
PMID: 19761218

Function and Biology Details

Reaction catalysed:
ADP-alpha-D-glucose + ((1->4)-alpha-D-glucosyl)(n) = ADP + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (5 distinct):
Glycogen synthase Chain: A
Molecule details ›
Chain: A
Length: 485 amino acids
Theoretical weight: 53.89 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6U8 (Residues: 1-477; Coverage: 100%)
Gene names: JW3392, b3429, glgA
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer : NEW Components: GLC, BGC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: I41
Unit cell:
a: 126.324Å b: 126.324Å c: 152.334Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.221
Expression system: Escherichia coli