PDBe 3cw1

X-ray diffraction
5.49Å resolution

Crystal Structure of Human Spliceosomal U1 snRNP

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Entry contents:
9 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (10 distinct):
Small nuclear ribonucleoprotein Sm D3 Chains: D, S, T, U
Molecule details ›
Chains: D, S, T, U
Length: 126 amino acids
Theoretical weight: 13.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62318 (Residues: 1-126; Coverage: 100%)
Gene name: SNRPD3
Sequence domains: LSM domain
Small nuclear ribonucleoprotein-associated proteins B and B' Chains: A, H, I, J
Molecule details ›
Chains: A, H, I, J
Length: 174 amino acids
Theoretical weight: 18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P14678 (Residues: 1-174; Coverage: 73%)
Gene names: COD, SNRPB, SNRPB1
Sequence domains: LSM domain
Small nuclear ribonucleoprotein Sm D1 Chains: B, M, N, O
Molecule details ›
Chains: B, M, N, O
Length: 119 amino acids
Theoretical weight: 13.31 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62314 (Residues: 1-119; Coverage: 100%)
Gene name: SNRPD1
Sequence domains: LSM domain
Small nuclear ribonucleoprotein Sm D2 Chains: C, P, Q, R
Molecule details ›
Chains: C, P, Q, R
Length: 118 amino acids
Theoretical weight: 13.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62316 (Residues: 1-118; Coverage: 100%)
Gene names: SNRPD1, SNRPD2
Sequence domains: LSM domain
Small nuclear ribonucleoprotein F Chains: 1, 2, F, Z
Molecule details ›
Chains: 1, 2, F, Z
Length: 86 amino acids
Theoretical weight: 9.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62306 (Residues: 1-86; Coverage: 100%)
Gene names: PBSCF, SNRPF
Sequence domains: LSM domain
Small nuclear ribonucleoprotein E Chains: E, W, X, Y
Molecule details ›
Chains: E, W, X, Y
Length: 92 amino acids
Theoretical weight: 10.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62304 (Residues: 1-92; Coverage: 100%)
Gene name: SNRPE
Sequence domains: LSM domain
Small nuclear ribonucleoprotein G Chains: 3, 4, 5, G
Molecule details ›
Chains: 3, 4, 5, G
Length: 76 amino acids
Theoretical weight: 8.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62308 (Residues: 1-76; Coverage: 100%)
Gene names: PBSCG, SNRPG
Sequence domains: LSM domain
U1 small nuclear ribonucleoprotein 70 kDa Chains: 6, 7, 8, K
Molecule details ›
Chains: 6, 7, 8, K
Length: 216 amino acids
Theoretical weight: 25.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P08621 (Residues: 1-216; Coverage: 49%)
Gene names: RNPU1Z, RPU1, SNRNP70, SNRP70, U1AP1
Sequence domains:
U1 small nuclear ribonucleoprotein C Chains: 0, 9, L, l
Molecule details ›
Chains: 0, 9, L, l
Length: 77 amino acids
Theoretical weight: 8.8 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09234 (Residues: 1-77; Coverage: 48%)
Gene name: SNRPC
Sequence domains: U1 zinc finger
U1 snRNA Chains: V, v, w, x
Molecule details ›
Chains: V, v, w, x
Length: 138 nucleotides
Theoretical weight: 44.42 KDa
Sequence domains: U1 spliceosomal RNA

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA, SLS BEAMLINE X10SA
Spacegroup: P1
Unit cell:
a: 126.471Å b: 127.075Å c: 152.024Å
α: 95.42° β: 105.92° γ: 101.8°
Expression system: Escherichia coli