3cug

X-ray diffraction
1.68Å resolution

Cellulomonas fimi Xylanase/Cellulase Cex (Cf Xyn10A) in complex with cellotetraose-like isofagomine

Released:
Source organism: Cellulomonas fimi
Entry authors: Kuntz DA, Saul M, Rose DR

Function and Biology Details

Reactions catalysed:
Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exoglucanase/xylanase Chain: A
Molecule details ›
Chain: A
Length: 315 amino acids
Theoretical weight: 34.27 KDa
Source organism: Cellulomonas fimi
Expression system: Escherichia coli
UniProt:
  • Canonical: P07986 (Residues: 42-356; Coverage: 71%)
Gene names: cex, xynB
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41212
Unit cell:
a: 85.722Å b: 85.722Å c: 79.042Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.147 0.146 0.178
Expression system: Escherichia coli