3cu8

X-ray diffraction
2.4Å resolution

Impaired binding of 14-3-3 to Raf1 is linked to Noonan and LEOPARD syndrome

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 245 amino acids
Theoretical weight: 27.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63104 (Residues: 1-245; Coverage: 100%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
RAF proto-oncogene serine/threonine-protein kinase Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 9 amino acids
Theoretical weight: 1.08 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P04049 (Residues: 256-264; Coverage: 1%)
Gene names: RAF, RAF1

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 72.4Å b: 83.84Å c: 111.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 0.188 0.259
Expression systems:
  • Escherichia coli
  • Not provided