3cu0

X-ray diffraction
1.9Å resolution

human beta 1,3-glucuronyltransferase I (GlcAT-I) in complex with UDP and GAL-GAL(6-SO4)-XYL(2-PO4)-O-SER

Released:

Function and Biology Details

Reaction catalysed:
UDP-alpha-D-glucuronate + [protein]-3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-serine = UDP + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 281 amino acids
Theoretical weight: 31.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O94766 (Residues: 76-335; Coverage: 78%)
Gene name: B3GAT3
Sequence domains: Glycosyltransferase family 43
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

Carbohydrate polymer : NEW Components: GAL
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21
Unit cell:
a: 57.047Å b: 48.399Å c: 103.708Å
α: 90° β: 92.4° γ: 90°
R-values:
R R work R free
0.21 0.21 0.234
Expression system: Escherichia coli BL21(DE3)