3ctr

X-ray diffraction
2.1Å resolution

Crystal structure of the RRM-domain of the poly(A)-specific ribonuclease PARN bound to m7GTP

Released:

Function and Biology Details

Reaction catalysed:
Exonucleolytic cleavage of poly(A) to 5'-AMP
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(A)-specific ribonuclease PARN Chain: A
Molecule details ›
Chain: A
Length: 101 amino acids
Theoretical weight: 11.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O95453 (Residues: 445-540; Coverage: 15%)
Gene names: DAN, PARN
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A, BESSY BEAMLINE 14.2
Spacegroup: I4122
Unit cell:
a: 81.107Å b: 81.107Å c: 78.058Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.21 0.243
Expression system: Escherichia coli BL21(DE3)