› 3cos

X-ray diffraction
2.1Å resolution

Crystal structure of human class II alcohol dehydrogenase (ADH4) in complex with NAD and Zn

Released:
DOI: 10.2210/pdb3cos/pdb
PDB entry 3cos coloured by chain, front view.
PDB entry 3cos coloured by chain, side view.
PDB entry 3cos coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Kavanagh KL, Shafqat N, Yue W, von Delft F, Bishop S, Roos A, Murray J, Edwards AM, Arrowsmith CH, Bountra C, Oppermann U, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
All-trans-retinol-[cellular-retinol-binding-protein] + NAD(+) = all-trans-retinal-[cellular-retinol-binding-protein] + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140097 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
All-trans-retinol dehydrogenase [NAD(+)] ADH4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 381 amino acids
Theoretical weight: 40.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P08319 (Residues: 1-380; Coverage: 100%)
Gene names: ADH2, ADH4
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 4 x NAD
3 bound ligands:
Ligand ZN 8 x ZN
Ligand EDO 7 x EDO
Ligand ACY 5 x ACY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P21
Unit cell:
a: 67.299Å b: 160.919Å c: 88.739Å
α: 90° β: 111.64° γ: 90°
R-values:
R R work R free
0.17 0.169 0.206
Expression system: Escherichia coli BL21(DE3)

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