3cmq

X-ray diffraction
2.2Å resolution

Crystal structure of human mitochondrial phenylalanine tRNA synthetase

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenylalanine--tRNA ligase, mitochondrial Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 48.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95363 (Residues: 38-451; Coverage: 92%)
Gene names: FARS1, FARS2, HSPC320
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P212121
Unit cell:
a: 54.888Å b: 90.168Å c: 95.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.259 0.192 0.242
Expression system: Escherichia coli