3cm8

X-ray diffraction
2.9Å resolution

A RNA polymerase subunit structure from virus

Released:

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Polymerase acidic protein Chain: A
Molecule details ›
Chain: A
Length: 471 amino acids
Theoretical weight: 53.87 KDa
Source organism: Influenza A virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EA60 (Residues: 256-716; Coverage: 64%)
Gene name: PA
Sequence domains: Influenza RNA-dependent RNA polymerase subunit PA
RNA-directed RNA polymerase catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 30 amino acids
Theoretical weight: 3.19 KDa
Source organism: Influenza A virus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WLS3 (Residues: 1-25; Coverage: 3%)
Gene name: PB1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID, RIGAKU FR-E+ DW
Spacegroup: P41212
Unit cell:
a: 121.942Å b: 121.942Å c: 134.594Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.224 0.261
Expression system: Escherichia coli