› 3clb

X-ray diffraction
3Å resolution

Function and Biology Details

Reactions catalysed: 
5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH
5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional dihydrofolate reductase-thymidylate synthase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 521 amino acids
Theoretical weight: 58.9 KDa
Source organism: Trypanosoma cruzi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q27793 (Residues: 1-521; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 4 x NAP
3 bound ligands:
Ligand SO4 4 x SO4
Ligand TMQ 4 x TMQ
Ligand EDO 6 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P43212
Unit cell:
a: 176.571Å b: 176.571Å c: 251.848Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.245
Expression system: Escherichia coli

Quick links

Citations

3 review citations

New, improved treatments for Chagas disease: from the R&D pipeline to the patients.
Ribeiro et al. (2009)
2 more

3 mentions without citation

Antifolate agents: a patent review (2006 - 2010).
Wright et al. (2011)
2 more