› 3cku

X-ray diffraction
1.7Å resolution

Urate oxidase from aspergillus flavus complexed with its inhibitor 8-azaxanthin and chloride

Released:
DOI: 10.2210/pdb3cku/pdb
PDB entry 3cku coloured by chain, front view.
PDB entry 3cku coloured by chain, side view.
PDB entry 3cku coloured by chain, top view.
Source organism: Aspergillus flavus
Primary publication:
Oxygen pressurized X-ray crystallography: probing the dioxygen binding site in cofactorless urate oxidase and implications for its catalytic mechanism.
Colloc'h N, Gabison L, Monard G, Altarsha M, Chiadmi M, Marassio G, Sopkova-de Oliveira Santos J, El Hajji M, Castro B, Abraini JH, Prangé T
Biophys J 95 2415-22 (2008)
PMID: 18375516

Function and Biology Details

Reaction catalysed: 
Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169432 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uricase Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 34.18 KDa
Source organism: Aspergillus flavus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q00511 (Residues: 2-302; Coverage: 100%)
Gene names: Uox, uaZ
Sequence domains: Uricase
Structure domains: Urate Oxidase;

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand NA 1 x NA
Ligand AZA 1 x AZA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: I222
Unit cell:
a: 78.994Å b: 94.979Å c: 104.381Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.193 0.251
Expression system: Saccharomyces cerevisiae

Quick links

Citations

3 review citations

Cofactor-independent oxidases and oxygenases.
Fetzner et al. (2010)
2 more

2 mentions without citation

Oxygen pressurized X-ray crystallography: probing the dioxygen binding site in cofactorless urate oxidase and implications for its catalytic mechanism.
Colloc'h et al. (2008)
1 more