› 3ckm

X-ray diffraction
1.35Å resolution

LpoA (YraM) C-domain from Haemophilus influenzae, a regulator of PBP1A

Released:
DOI: 10.2210/pdb3ckm/pdb
PDB entry 3ckm coloured by chain, front view.
PDB entry 3ckm coloured by chain, side view.
PDB entry 3ckm coloured by chain, top view.
Source organism: Haemophilus influenzae Rd KW20
Primary publication:
Structure of YraM, a protein essential for growth of Haemophilus influenzae.
Vijayalakshmi J, Akerley BJ, Saper MA
Proteins 73 204-17 (2008)
PMID: 18412262

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155267 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Penicillin-binding protein activator LpoA Chain: A
Molecule details ›
Chain: A
Length: 327 amino acids
Theoretical weight: 36.23 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli
UniProt:
  • Canonical: P45299 (Residues: 256-573; Coverage: 58%)
Gene names: HI_1655, lpoA
Sequence domains: LppC putative lipoprotein
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand BME 1 x BME
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: C2
Unit cell:
a: 109.907Å b: 50.644Å c: 63.676Å
α: 90° β: 106.73° γ: 90°
R-values:
R R work R free
0.16 0.157 0.194
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Do the divisome and elongasome share a common evolutionary past?
Szwedziak et al. (2013)
1 more

3 mentions without citation

Elongated structure of the outer-membrane activator of peptidoglycan synthesis LpoA: implications for PBP1A stimulation.
Jean et al. (2014)
2 more