X-ray diffraction
2.3Å resolution

Complex of Dictyostelium discoideum Actin with Profilin and the Last Poly-Pro of Human VASP

Primary publication:
Modulation of actin structure and function by phosphorylation of Tyr-53 and profilin binding.
Proc. Natl. Acad. Sci. U.S.A. 105 11748-53 (2008)
PMID: 18689676

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Major actin Chain: A
Profilin-1 Chain: P
Molecule details ›
Chain: P
Length: 139 amino acids
Theoretical weight: 14.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P07737 (Residues: 2-140; Coverage: 99%)
Gene name: PFN1
Sequence domains: Profilin
Structure domains: Dynein light chain 2a, cytoplasmic
Vasodilator-stimulated phosphoprotein Chain: V
Molecule details ›
Chain: V
Length: 16 amino acids
Theoretical weight: 1.35 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P50552 (Residues: 199-214; Coverage: 4%)
Gene name: VASP

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F2
Spacegroup: P212121
Unit cell:
a: 38.037Å b: 76.183Å c: 180.822Å
α: 90° β: 90° γ: 90°
R R work R free
0.161 0.157 0.227
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided