› 3cb2

X-ray diffraction
2.3Å resolution

Crystal structure of human gamma-tubulin bound to GDP

Released:
DOI: 10.2210/pdb3cb2/pdb
PDB entry 3cb2 coloured by chain, front view.
PDB entry 3cb2 coloured by chain, side view.
PDB entry 3cb2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The lattice as allosteric effector: structural studies of alphabeta- and gamma-tubulin clarify the role of GTP in microtubule assembly.
Rice LM, Montabana EA, Agard DA
Proc Natl Acad Sci U S A 105 5378-83 (2008)
PMID: 18388201

Function and Biology Details

Reaction catalysed: 
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149915 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tubulin gamma-1 chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 475 amino acids
Theoretical weight: 54.04 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P23258 (Residues: 1-451; Coverage: 100%)
Gene names: TUBG, TUBG1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P21
Unit cell:
a: 51.744Å b: 108.755Å c: 83.347Å
α: 90° β: 96.33° γ: 90°
R-values:
R R work R free
0.189 0.187 0.238
Expression system: Spodoptera frugiperda

Quick links

Citations

27 review citations

Microtubule nucleation by γ-tubulin complexes.
Kollman et al. (2011)
26 more

21 mentions without citation

Human ES cell-derived neural rosettes reveal a functionally distinct early neural stem cell stage.
Elkabetz et al. (2008)
20 more