X-ray diffraction
1.5Å resolution

Crystal structure of the uncharacterized human protein C8orf32 with bound peptide

Source organism: Homo sapiens
Entry authors: Bitto E, Bingman CA, McCoy JG, Wesenberg GE, Phillips Jr GN, Center for Eukaryotic Structural Genomics (CESG)

Function and Biology Details

Reaction catalysed:
N-terminal L-glutaminyl-[protein] + H(2)O = N-terminal L-glutamyl-[protein] + NH(3)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein N-terminal glutamine amidohydrolase Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 23.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q96HA8 (Residues: 2-205; Coverage: 100%)
Gene names: C8orf32, NTAQ1, WDYHV1
Sequence domains: N-terminal glutamine amidase
Structure domains: Protein N-terminal glutamine amidohydrolase, alpha beta roll
Synthetic peptide Chain: L
Molecule details ›
Chain: L
Length: 3 amino acids
Theoretical weight: 277 Da
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 34.322Å b: 64.039Å c: 113.66Å
α: 90° β: 90° γ: 90°
R R work R free
0.162 0.161 0.18
Expression systems:
  • Escherichia coli
  • Not provided