› 3c7k

X-ray diffraction
2.9Å resolution

Molecular architecture of Galphao and the structural basis for RGS16-mediated deactivation

Released:
DOI: 10.2210/pdb3c7k/pdb
PDB entry 3c7k coloured by chain, front view.
PDB entry 3c7k coloured by chain, side view.
PDB entry 3c7k coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Molecular architecture of Galphao and the structural basis for RGS16-mediated deactivation.
Slep KC, Kercher MA, Wieland T, Chen CK, Simon MI, Sigler PB
Proc Natl Acad Sci U S A 105 6243-8 (2008)
PMID: 18434540

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148459 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(o) subunit alpha Chains: A, C
Molecule details ›
Chains: A, C
Length: 333 amino acids
Theoretical weight: 37.85 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P18872 (Residues: 22-354; Coverage: 94%)
Gene names: Gna0, Gnao, Gnao1
Sequence domains: G-protein alpha subunit
Structure domains:
Regulator of G-protein signaling 16 Chains: B, D
Molecule details ›
Chains: B, D
Length: 129 amino acids
Theoretical weight: 14.86 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P97428 (Residues: 53-180; Coverage: 64%)
Gene names: Rgs16, Rgsr
Sequence domains: Regulator of G protein signaling domain
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 2 x MG
Ligand ALF 2 x ALF
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P3221
Unit cell:
a: 96.357Å b: 96.357Å c: 235.61Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.25 0.25 0.311
Expression system: Escherichia coli BL21

Quick links

Citations

6 review citations

Regulators of G-protein signaling and their Gα substrates: promises and challenges in their use as drug discovery targets.
Kimple et al. (2011)
5 more

12 mentions without citation

Functions of regulators of G protein signaling 16 in immunity, inflammation, and other diseases.
Tian et al. (2022)
11 more