› 3c4b

X-ray diffraction
1.68Å resolution

Structure of RNaseIIIb and dsRNA binding domains of mouse Dicer

Released:
DOI: 10.2210/pdb3c4b/pdb
PDB entry 3c4b coloured by chain, front view.
PDB entry 3c4b coloured by chain, side view.
PDB entry 3c4b coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural and biochemical insights into the dicing mechanism of mouse Dicer: a conserved lysine is critical for dsRNA cleavage.
Du Z, Lee JK, Tjhen R, Stroud RM, James TL
Proc Natl Acad Sci U S A 105 2391-6 (2008)
PMID: 18268334

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endoribonuclease Dicer Chain: A
Molecule details ›
Chain: A
Length: 265 amino acids
Theoretical weight: 30.97 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8R418 (Residues: 1648-1910; Coverage: 14%)
Gene names: Dicer, Dicer1, Mdcr
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
2 modified residues:
Ligand OCS 1 x OCS
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P42212
Unit cell:
a: 66.496Å b: 66.496Å c: 134.081Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.18 0.21
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

A three-dimensional view of the molecular machinery of RNA interference.
Jinek et al. (2009)
10 more

14 mentions without citation

RNA recognition by double-stranded RNA binding domains: a matter of shape and sequence.
Masliah et al. (2013)
13 more