3c1h

X-ray diffraction
2.2Å resolution

Substrate binding, deprotonation and selectivity at the periplasmic entrance of the E. coli ammonia channel AmtB

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ammonia channel Chain: A
Molecule details ›
Chain: A
Length: 424 amino acids
Theoretical weight: 44.38 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69681 (Residues: 23-428; Coverage: 100%)
Gene names: JW0441, amtB, b0451, ybaG
Sequence domains: Ammonium Transporter Family
Structure domains: Ammonium transporter AmtB like domains

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P63
Unit cell:
a: 108.175Å b: 108.175Å c: 84.663Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.204 0.231
Expression system: Escherichia coli