3c0k

X-ray diffraction
2Å resolution

Crystal Structure of a ribosomal RNA methyltranferase

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + cytosine(1962) in 23S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(1962) in 23S rRNA
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA large subunit methyltransferase I Chains: A, B
Molecule details ›
Chains: A, B
Length: 396 amino acids
Theoretical weight: 44.88 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P75876 (Residues: 1-396; Coverage: 100%)
Gene names: JW5898, b0967, rlmI, yccW
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 64.312Å b: 80.875Å c: 66.155Å
α: 90° β: 98.91° γ: 90°
R-values:
R R work R free
0.194 0.194 0.242
Expression system: Escherichia coli