› 3bzo

X-ray diffraction
1.5Å resolution

Crystal structural of native EscU C-terminal domain

Released:
DOI: 10.2210/pdb3bzo/pdb
PDB 3bzo coloured by chain and viewed from the front.
PDB 3bzo coloured by chain and viewed from the side.
PDB 3bzo coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Structural analysis of the essential self-cleaving type III secretion proteins EscU and SpaS.
Zarivach R, Deng W, Vuckovic M, Felise HB, Nguyen HV, Miller SI, Finlay BB, Strynadka NC
Nature 453 124-7 (2008)
PMID: 18451864

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Type III secretion system LEE export apparatus switch protein EscU Chain: A
Molecule details ›
Chain: A
Length: 54 amino acids
Theoretical weight: 5.99 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AJ26 (Residues: 215-262; Coverage: 14%)
Gene names: BG944_004520, C0P57_005208, CTR35_004860, CX694_003193, EAN77_23705, EAX79_25000, HKA49_004483, NCTC8009_02025, NCTC8179_06337, NCTC8960_02103, NCTC9111_01230, escU
Type III secretion system LEE export apparatus switch protein EscU Chain: B
Molecule details ›
Chain: B
Length: 83 amino acids
Theoretical weight: 9.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9AJ26 (Residues: 263-345; Coverage: 24%)
Gene names: BG944_004520, C0P57_005208, CTR35_004860, CX694_003193, EAN77_23705, EAX79_25000, HKA49_004483, NCTC8009_02025, NCTC8179_06337, NCTC8960_02103, NCTC9111_01230, escU
Structure domains: secretion proteins EscU

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: I222
Unit cell:
a: 55.692Å b: 58.708Å c: 72.605Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.202 0.225
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Type three secretion system in Salmonella Typhimurium: the key to infection.
Dos Santos et al. (2020)
26 more

2 mentions without citation

Atomic resolution structure of the cytoplasmic domain of Yersinia pestis YscU, a regulatory switch involved in type III secretion.
Lountos et al. (2009)
1 more