X-ray diffraction
1.8Å resolution

Crystal structure of uncharacterized metallo protein from Vibrio cholerae with beta-lactamase like fold

Entry authors: Minasov G, Shuvalova L, Brunzelle JS, Yang X, Collart FR, Joachimiak A, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Metal-dependent hydrolase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 379 amino acids
Theoretical weight: 43.25 KDa
Source organism: Vibrio cholerae O1 biovar El Tor str. N16961
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9KMS2 (Residues: 1-355; Coverage: 100%)
Gene name: VC_A0248
Sequence domains: Beta-lactamase superfamily domain
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: P1
Unit cell:
a: 83.433Å b: 94.839Å c: 94.591Å
α: 99.69° β: 97.9° γ: 116.13°
R R work R free
0.169 0.167 0.208
Expression system: Escherichia coli BL21(DE3)