› 3bs8

X-ray diffraction
2.3Å resolution

Crystal structure of Glutamate 1-Semialdehyde Aminotransferase complexed with pyridoxamine-5'-phosphate From Bacillus subtilis

Released:
DOI: 10.2210/pdb3bs8/pdb
PDB entry 3bs8 coloured by chain, front view.
PDB entry 3bs8 coloured by chain, side view.
PDB entry 3bs8 coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structure of glutamate-1-semialdehyde aminotransferase from Bacillus subtilis with bound pyridoxamine-5'-phosphate.
Ge H, Lv X, Fan J, Gao Y, Teng M, Niu L
Biochem Biophys Res Commun 402 356-60 (2010)
PMID: 20946885

Function and Biology Details

Reaction catalysed: 
(S)-4-amino-5-oxopentanoate = 5-aminolevulinate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151849 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate-1-semialdehyde 2,1-aminomutase Chain: A
Molecule details ›
Chain: A
Length: 438 amino acids
Theoretical weight: 47.52 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P30949 (Residues: 1-430; Coverage: 100%)
Gene names: BSU28120, hemK, hemL
Sequence domains: Aminotransferase class-III
Structure domains:

Ligands and Environments


Cofactor: Ligand PMP 1 x PMP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 109.251Å b: 55.761Å c: 80.504Å
α: 90° β: 132.02° γ: 90°
R-values:
R R work R free
0.195 0.193 0.234
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Prokaryotic Heme Biosynthesis: Multiple Pathways to a Common Essential Product.
Dailey et al. (2017)
2 more

5 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
4 more