3brw

X-ray diffraction
3.4Å resolution

Structure of the Rap-RapGAP complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rap1 GTPase-activating protein 1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 341 amino acids
Theoretical weight: 38.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P47736 (Residues: 75-415; Coverage: 51%)
Gene names: KIAA0474, RAP1GA1, RAP1GAP
Sequence domains: Rap/ran-GAP
Structure domains: Rossmann fold
Ras-related protein Rap-1b Chain: D
Molecule details ›
Chain: D
Length: 167 amino acids
Theoretical weight: 19.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61224 (Residues: 1-167; Coverage: 91%)
Gene names: OK/SW-cl.11, RAP1B
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3121
Unit cell:
a: 209.723Å b: 209.723Å c: 108.217Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.234 0.28
Expression system: Escherichia coli BL21(DE3)