3bp3

X-ray diffraction
1.65Å resolution

Crystal structure of EIIB

Released:

Function and Biology Details

Reaction catalysed:
[Protein]-N(pi)-phospho-L-histidine + D-glucose(Side 1) = [protein]-L-histidine + D-glucose 6-phosphate(Side 2)

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system glucose-specific EIICB component Chains: A, B
Molecule details ›
Chains: A, B
Length: 82 amino acids
Theoretical weight: 8.58 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69786 (Residues: 396-477; Coverage: 17%)
Gene names: JW1087, b1101, glcA, ptsG, umg
Sequence domains: phosphotransferase system, EIIB
Structure domains: Glucose permease domain IIB

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: C2221
Unit cell:
a: 108.193Å b: 108.149Å c: 28.947Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.241
Expression system: Escherichia coli