› 3bf8

X-ray diffraction
1.68Å resolution

1.1 resolution structure of ybfF, a new esterase from Escherichia coli: a unique substrate-binding crevice generated by domain arrangement

Released:
DOI: 10.2210/pdb3bf8/pdb
PDB entry 3bf8 coloured by chain, front view.
PDB entry 3bf8 coloured by chain, side view.
PDB entry 3bf8 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
High-resolution structure of ybfF from Escherichia coli K12: a unique substrate-binding crevice generated by domain arrangement.
Park SY, Lee SH, Lee J, Nishi K, Kim YS, Jung CH, Kim JS
J Mol Biol 376 1426-37 (2008)
PMID: 18215690

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159992 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Esterase YbfF Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 28.6 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P75736 (Residues: 1-254; Coverage: 100%)
Gene names: JW0673, b0686, ybfF
Sequence domains: Alpha/beta hydrolase family
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand MLA 2 x MLA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 6C1
Spacegroup: P212121
Unit cell:
a: 66.216Å b: 91.061Å c: 93.253Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.216 0.24
Expression system: Escherichia coli

Quick links

Citations

15 citation in other articles

Characterization of a novel esterase Rv0045c from Mycobacterium tuberculosis.
Guo et al. (2010)
14 more

4 mentions without citation

Carboxylic ester hydrolases: Classification and database derived from their primary, secondary, and tertiary structures.
Chen et al. (2016)
3 more