3bdp

X-ray diffraction
1.9Å resolution

DNA POLYMERASE I/DNA COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 66.2 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: P52026 (Residues: 297-876; Coverage: 66%)
Gene names: pol, polA
Sequence domains: DNA polymerase family A
Structure domains:
DNA (5'-D(*GP*CP*AP*TP*GP*AP*TP*GP*CP*2DT)-3') Chain: P
Molecule details ›
Chain: P
Length: 10 nucleotides
Theoretical weight: 3.04 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided
DNA (5'-D(*AP*GP*CP*AP*TP*CP*AP*TP*GP*C)-3') Chain: T
Molecule details ›
Chain: T
Length: 10 nucleotides
Theoretical weight: 3.03 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 87.063Å b: 93.547Å c: 106.252Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.25 0.294
Expression systems:
  • Escherichia coli
  • Not provided