Protein Data Bank in Europe

Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val

Chloride + O(2) = chlorite

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin

4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O

4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O

2 5-aminolevulinate = porphobilinogen + 2 H(2)O

L-glutamate = D-glutamate

Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine

ATP + RNA(n) = diphosphate + RNA(n+1)

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

D-glyceraldehyde 3-phosphate = glycerone phosphate

L-aspartate = D-aspartate

An L-amino acid = a D-amino acid

L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH

S-formylglutathione + H(2)O = glutathione + formate

S-methyl-5'-thioadenosine + H(2)O = 5-(methylsulfanyl)-D-ribose + adenine

Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.

D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H(2)O

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O

(2E,6E)-farnesyl diphosphate = amorpha-4,11-diene + diphosphate

N(6)-prenyladenine + acceptor + H(2)O = adenine + 3-methylbut-2-enal + reduced acceptor

ATP + kanamycin = ADP + kanamycin 3'-phosphate

Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.

ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)

N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA

(1a) L-tyrosine + 1/2 O(2) = L-dopa

3-dehydroquinate = 3-dehydroshikimate + H(2)O

dTDP-beta-L-rhamnose + NADP(+) = dTDP-4-dehydro-beta-L-rhamnose + NADPH

Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]

NTP + H(2)O = NDP + phosphate

ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA

A chalcone = a flavanone

4 ferrocytochrome c + O(2) + 4 H(+) = 4 ferricytochrome c + 2 H(2)O

RX + glutathione = HX + R-S-glutathione

S-adenosyl 3-(methylthio)propylamine + putrescine = 5'-S-methyl-5'-thioadenosine + spermidine

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans

(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH

(R)-3-hydroxyacyl-CoA + NADP(+) = 3-oxoacyl-CoA + NADPH

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate

S-adenosyl-L-homocysteine + H(2)O = L-homocysteine + adenosine

Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin

Methyl-CoM + CoB = CoM-S-S-CoB + methane

ATP + glycerol = ADP + sn-glycerol 3-phosphate

ATP + pyridoxal = ADP + pyridoxal 5'-phosphate

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O

5'-deoxyadenosine + H(2)O = 5-deoxy-D-ribose + adenine

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate

H(2)CO(3) = CO(2) + H(2)O

dUTP + H(2)O = dUMP + diphosphate

Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate 

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains

ATP + a protein = ADP + a phosphoprotein

Isochorismate + H(2)O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate

Formate + NAD(+) = CO(2) + NADH

Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)

NH(3) + 2 H(2)O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H(+)

N-acetyl-O-acetylneuraminate + H(2)O = N-acetylneuraminate + acetate

Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH

L-fucopyranose = L-fuculose

Benzoylformate = benzaldehyde + CO(2)

S-adenosyl-L-methionine + 3-((1-carboxyvinyl)oxy)benzoate + H(2)O = 6-amino-6-deoxyfutalosine + L-methionine + HCO(3)(-)

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH

A beta-lactam + H(2)O = a substituted beta-amino acid

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

Peptidylproline (omega=180) = peptidylproline (omega=0)

D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH

Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

(1a) cyanate + HCO(3)(-) + H(+) = carbamate + CO(2)

(S)-lactate + NAD(+) = pyruvate + NADH

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)

ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide

ATP + H(2)O = ADP + phosphate

5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH

ATP + propanoyl-CoA + HCO(3)(-) = ADP + phosphate + (S)-methylmalonyl-CoA

H(2) + A = AH(2)

Diphosphate + H(2)O = 2 phosphate

Maltose = alpha,alpha-trehalose

An aliphatic amide = a nitrile + H(2)O

Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine

L-cystathionine + H(2)O = L-homocysteine + NH(3) + pyruvate

(1a) L-cystathionine = L-cysteine + 2-aminobut-2-enoate

(R)-S-lactoylglutathione = glutathione + methylglyoxal

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins

NAD(+) + glycine + sulfide = nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + 3 H(2)O

5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA

Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate

2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O

A (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein] + CO(2) + an [acyl-carrier protein]

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose

(1a) L-glutamine + H(2)O = L-glutamate + NH(3)

Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-|-Xaa- and -Yaa-Xaa-Gly-Xaa-|-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid).

Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil

H(2) + NAD(+) = H(+) + NADH

Acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N(3)-acetyl-2-deoxystreptamine antibiotic

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides

L-ornithine = L-proline + NH(3)

ATP + L-pyrrolysine + tRNA(Pyl) = AMP + diphosphate + L-pyrrolysyl-tRNA(Pyl)

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides

2 superoxide + 2 H(+) = O(2) + H(2)O(2)

Hydrolysis of proteins in presence of ATP.

Phosphoenolpyruvate + N-acetyl-D-mannosamine + H(2)O = phosphate + N-acetylneuraminate

N-carbamoylputrescine + H(2)O = putrescine + CO(2) + NH(3)

Prephenate + NADP(+) = 4-hydroxyphenylpyruvate + CO(2) + NADPH

(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide

A pyrimidine 5'-nucleotide + H(2)O = D-ribose 5-phosphate + a pyrimidine base

(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine

L-serine + L-homocysteine = L-cystathionine + H(2)O

Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH

ATP + beta-nicotinate-D-ribonucleotide = diphosphate + deamido-NAD(+)

[Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2)

ATP + UMP = ADP + UDP

2 S-adenosyl-L-methionine + adenine(2503) in 23S rRNA + 2 reduced [2Fe-2S] ferredoxin = S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine(2503) in 23S rRNA + 2 oxidized [2Fe-2S] ferredoxin

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

S-adenosyl-L-methionine + adenine(22) in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(22) in tRNA

A glutathione-S-conjugate + H(2)O = an (L-cysteinylglycine)-S-conjugate + L-glutamate

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

L-carnitine + NAD(P)H + O(2) = (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD(P)(+) + H(2)O

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate

A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + a UDP-3-O-((3R)-hydroxyacyl)-alpha-D-glucosamine = a UDP-2-N,3-O-bis((3R)-3-hydroxyacyl)-alpha-D-glucosamine + a holo-[acyl-carrier-protein]

2 nitric oxide + 2 O(2) + NAD(P)H = 2 nitrate + NAD(P)(+) + H(+)

Betaine aldehyde + NAD(+) + H(2)O = betaine + NADH

Produces alpha-D-fructofuranose beta-D-fructofuranose 1,2':2,1'-dianhydride (DFA I) by successively eliminating the diminishing (2->1)-beta-D-fructan (inulin) chain from the terminal D-fructosyl-D-fructosyl disaccharide.

sn-glycero-3-phosphocholine + H(2)O = choline + sn-glycerol 3-phosphate

ATP + (L-Asp(4-L-Arg))(n) + L-Asp = ADP + phosphate + (L-Asp(4-L-Arg))(n)-L-Asp

Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate

A beta-D-glucuronoside + H(2)O = D-glucuronate + an alcohol

Hydrolysis of (1->4)-beta-D-xylans, to remove successive D-xylose residues from the non-reducing termini

1-(5-phospho-beta-D-ribosyl)-AMP + H(2)O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide

(1a) glutaryl-CoA + electron-transfer flavoprotein = (E)-glutaconyl-CoA + reduced electron-transfer flavoprotein

2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate

Urea + H(2)O = CO(2) + 2 NH(3)

(1a) (2R,3S)-3-isopropylmalate + NAD(+) = (2S)-2-isopropyl-3-oxosuccinate + NADH

FAD + [protein]-L-threonine = [protein]-FMN-L-threonine + AMP

TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.

1-haloalkane + H(2)O = a primary alcohol + halide

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.

Transfers a segment of a (1->4)-alpha-D-glucan to a new position in an acceptor, which may be glucose or a (1->4)-alpha-D-glucan

(1a) [enzyme]-L-histidine + 2,3-bisphospho-D-glycerate = [enzyme]-N(tau)-phospho-L-histidine + 2/3-phospho-D-glycerate

2 H(2)O(2) = O(2) + 2 H(2)O

S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-ribonucleotide)-[mRNA]

(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + NADH

D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate

Prenyl phosphate + FMNH(2) = prenylated FMNH(2) + phosphate

6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO(2) + NADPH

A primary alcohol + 2 cytochrome c(L) = an aldehyde + 2 reduced cytochrome c(L) + 2 H(+)

A primary alcohol + 2 cytochrome c = an aldehyde + 2 reduced cytochrome c + 2 H(+)

(1a) N(6)-(3-methylbut-2-en-1-yl)-adenine(37) in tRNA + sulfur-(sulfur carrier) + S-adenosyl-L-methionine + reduced electron acceptor = N(6)-(3-methylbut-2-en-1-yl)-2-thioadenine(37) in tRNA + (sulfur carrier) + L-methionine + 5'-deoxyadenosine + electron acceptor

Beta-D-ribopyranose = beta-D-ribofuranose

Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside

A purine nucleoside + H(2)O = D-ribose + a purine base

An acyl-[acyl-carrier protein] + NADP(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH

Arachidonate + O(2) = (5Z,8Z,11Z,13E)-(15S)-15-hydroperoxyicosa-5,8,11,13-tetraenoate

2 ATP = 2 diphosphate + cyclic di-3',5'-adenylate

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate

AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate

UDP-N-acetyl-alpha-D-glucosamine = UDP-2-acetamido-2,6-dideoxy-alpha-D-xylo-hex-4-ulose + H(2)O

Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose

Hydrolysis of (1->4)-alpha-D-glucosidic linkages in amylaceous polysaccharides, to remove successive maltohexaose residues from the non-reducing chain ends

Aceneneuramate = N-acetyl-D-mannosamine + pyruvate

A short-chain acyl-CoA + electron-transfer flavoprotein = a short-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

2 bilirubin + O(2) = 2 biliverdin + 2 H(2)O

Coproporphyrinogen-III + O(2) + 2 H(+) = protoporphyrinogen-IX + 2 CO(2) + 2 H(2)O

A nitroalkane + H(2)O + O(2) = an aldehyde or ketone + nitrite + H(2)O(2)

L-aspartate 4-semialdehyde + phosphate + NADP(+) = L-4-aspartyl phosphate + NADPH

Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan, amylopectin and glycogen, and in the alpha- and beta-limit dextrins of amylopectin and glycogen.

Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol

(R)-lactate + NAD(+) = pyruvate + NADH

Succinate + a quinone = fumarate + a quinol

A phosphate monoester + H(2)O = an alcohol + phosphate

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = an [acyl-carrier-protein] + a UDP-3-O-(3-hydroxyacylyl)-N-acetyl-alpha-D-glucosamine

Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.

4 sulfur + 4 H(2)O + O(2) = 2 H(2)S + 2 HSO(3)(-) + 2 H(+)

Cleavage of peptide bonds with very broad specificity.

Catechol + O(2) = cis,cis-muconate

Oligoglycosylglucosyl-(1<->1)-ceramide + H(2)O = ceramide + oligoglycosylglucose

Release of N-terminal proline from a peptide.

10-formyltetrahydrofolate + H(2)O = formate + tetrahydrofolate

L-allo-threonine = glycine + acetaldehyde

1-(5-phospho-beta-D-ribosyl)-ATP + H(2)O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate

L-lysine + NADPH + O(2) = N(6)-hydroxy-L-lysine + NADP(+) + H(2)O

Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH

Formyl-L-methionyl peptide + H(2)O = formate + methionyl peptide

5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate

3-deoxy-D-manno-octulosonate 8-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate + phosphate

2 reduced ferredoxin + NADP(+) + H(+) = 2 oxidized ferredoxin + NADPH

Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.

Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.

(R)-2-haloacid + H(2)O = (S)-2-hydroxyacid + halide

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans

Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units

ADP-alpha-D-glucose + D-glucose 6-phosphate = ADP + alpha,alpha-trehalose 6-phosphate

Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.

Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]

ATP + (L-Asp(4-L-Arg))(n)-L-Asp + L-Arg = ADP + phosphate + (L-Asp(4-L-Arg))(n+1)

ATP + H(2)O + K(+)(Side 1) = ADP + phosphate + K(+)(Side 2)

RH + H(2)O(2) = ROH + H(2)O

A (5-L-glutamyl)-peptide + an amino acid = a peptide + a 5-L-glutamyl amino acid

Endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor.

Endonucleolytic cleavage to 5'-phosphomonoester.

(S)-2-hydroxy acid + O(2) = 2-oxo acid + H(2)O(2)

Cyclic pyranopterin phosphate + 2 [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + H(2)O = molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]

Trypanothione + NADP(+) = trypanothione disulfide + NADPH

L-lysine + O(2) + H(2)O = 6-amino-2-oxohexanoate + NH(3) + H(2)O(2)

ATP + 3-methylbut-3-en-1-yl phosphate = ADP + 3-methylbut-3-en-1-yl diphosphate

(1a) S-adenosyl-L-methionine + L-histidine = S-adenosyl-L-homocysteine + N(alpha)-methyl-L-histidine

D-xylopyranose = D-xylulose

Cutin + H(2)O = cutin monomers

2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde

ATP + AMP = 2 ADP

ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate

(+)-trans-carveol + NAD(+) = (+)-(S)-carvone + NADH

Benzoate + NADH + O(2) = (1R,6S)-1,6-dihydroxycyclohexa-2,4-diene-1-carboxylate + NAD(+)

An aldehyde + NAD(P)(+) + H(2)O = a carboxylate + NAD(P)H

(1a) (S)-6-hydroxynicotine + O(2) = 5-(N-methyl-4,5-dihydro-1H-pyrrol-2-yl)pyridin-2-ol + H(2)O(2)

3-phospho-D-glycerate + NAD(+) = 3-phosphonooxypyruvate + NADH

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

L-asparagine + H(2)O = L-aspartate + NH(3)

(1a) S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine

ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N(6)-biotinyl-L-lysine

(+)-muconolactone = cis,cis-muconate

6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate

L-alanine = D-alanine

Release of a C-terminal amino acid with broad specificity, except for -Pro.

(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends

L-glutamate = 4-aminobutanoate + CO(2)

L-arginine + 2-oxoglutarate + O(2) = (3S)-3-hydroxy-L-arginine + succinate + CO(2)

Acetyl-CoA + H(2)O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain

(S)-mandelate = (R)-mandelate

5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2)

Cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate

Cleavage of hydrophobic, N-terminal signal or leader sequences from secreted and periplasmic proteins.

(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate

5,6-dihydrouracil + H(2)O = 3-ureidopropanoate

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate

ATP + pantetheine 4'-phosphate = diphosphate + 3'-dephospho-CoA

Maleate = fumarate

Geranylgeranyl diphosphate = (+)-copalyl diphosphate

GDP-mannose + 3-phospho-D-glycerate = GDP + 2-(alpha-D-mannosyl)-3-phosphoglycerate

Cytosine + H(2)O = uracil + NH(3)

NAD(P)H + a quinone = NAD(P)(+) + a hydroquinone

ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + L-threonyl-tRNA(Thr)

n ATP + n H(2)O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers

ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA

(1a) (2R,3S)-3-isopropylmalate = 2-isopropylmaleate + H(2)O

Triacylglycerol + H(2)O = diacylglycerol + a carboxylate

A carboxylic ester + H(2)O = an alcohol + a carboxylate

Endohydrolysis of (1->6)-alpha-D-glucosidic linkages in dextran

An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH

ATP = 3',5'-cyclic AMP + diphosphate

Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.

Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.

D-galactonate = 2-dehydro-3-deoxy-D-galactonate + H(2)O

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

ATP + [biotin carboxyl-carrier protein]-biotin-N(6)-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N(6)-L-lysine

(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate

[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine

CoB + CoM + methanophenazine = CoM-S-S-CoB + dihydromethanophenazine

A primary alcohol + NAD(+) = an aldehyde + NADH

Glycerone phosphate = methylglyoxal + phosphate

4-coumaroyl-CoA + shikimate = CoA + 4-coumaroylshikimate

2-deoxy-D-gluconate + NAD(+) = 3-dehydro-2-deoxy-D-gluconate + NADH

Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH

Methylamine + H(2)O + 2 oxidized [amicyanin] = formaldehyde + NH(3) + 2 reduced [amicyanin]

SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate

4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate

D-ribulose 5-phosphate = D-xylulose 5-phosphate

Release of an N-terminal dipeptide from a peptide comprising four or more residues, with broad specificity. Also acts on dipeptidyl 2-naphthylamides.

Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyzes peptide amides.

Endohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose in kappa-carrageenans.

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate

dTTP + alpha-D-glucose 1-phosphate = diphosphate + dTDP-alpha-D-glucose

3'-phosphoadenylyl sulfate + a phenol = adenosine 3',5'-bisphosphate + an aryl sulfate

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds

D-sorbitol 6-phosphate + NAD(+) = D-fructose 6-phosphate + NADH

(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine

3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid

Orotidine 5'-phosphate = UMP + CO(2)

D-glucosyl-N-acylsphingosine + H(2)O = D-glucose + N-acylsphingosine

NAD(P)H + O(2) = NAD(P)(+) + H(2)O(2)

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)

4-trimethylammoniobutanoate + 2-oxoglutarate + O(2) = 3-hydroxy-4-trimethylammoniobutanoate + succinate + CO(2) 

L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H

(S)(+)-allantoin = (R)(-)-allantoin

(1a) L-tryptophan = indole + 2-aminoprop-2-enoate

N-carbamoyl-L-2-amino acid (a 2-ureido carboxylate) + H(2)O = L-2-amino acid + NH(3) + CO(2)

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.

GTP = 3',5'-cyclic GMP + diphosphate

An aryl dialkyl phosphate + H(2)O = dialkyl phosphate + an aryl alcohol

[Dinitrogen reductase]-N(omega)-alpha-(ADP-D-ribosyl)-L-arginine = ADP-D-ribose + [dinitrogen reductase]-L-arginine

Angiotensin II + H(2)O = angiotensin-(1-7) + L-phenylalanine

2 glutathione + NADP(+) = glutathione disulfide + NADPH

Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)

Endohydrolysis of (1->4)-beta-D-galactosidic linkages in blood group A and B substances

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate

GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + NADPH

ATP-dependent breakage, passage and rejoining of double-stranded DNA

(2S)-2-hydroxy-3,4-dioxopentyl phosphate = 3-hydroxy-2,4-dioxopentyl phosphate

L-glutamine + 2,4,6/3,5-pentahydroxycyclohexanone = 2-oxoglutaramate + 1-amino-1-deoxy-scyllo-inositol

Triphosphate + [microsomal-membrane protein] = diphosphate + [microsomal-membrane protein] phosphate

(Ribonucleotide)(n)-2',3'-cyclic phosphate + H(2)O = (ribonucleotide)(n)-2'-phosphate

Prephenate = phenylpyruvate + H(2)O + CO(2)

(R)-10-hydroxystearate = oleate + H(2)O

(1a) L-glutamine + H(2)O = L-glutamate + NH(4)(+)

4-phosphooxy-L-threonine + NAD(+) = 3-amino-2-oxopropyl phosphate + CO(2) + NADH

Digestion of native collagen in the triple helical region at -|-Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1', Pro and Ala at P2 and P2', and hydroxyproline, Ala or Arg at P3'.

(1a) NAD(+) = cyclic ADP-ribose + nicotinamide

Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate

O-phospho-L-homoserine + H(2)O = L-threonine + phosphate

(1a) NADP(+) = 2'-phospho-cyclic ADP-ribose + nicotinamide

5-enolpyruvoyl-6-hydroxy-2-succinylcyclohex-3-ene-1-carboxylate = (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate + pyruvate

(R)-mandelonitrile = cyanide + benzaldehyde

Succinate semialdehyde + NAD(P)(+) + H(2)O = succinate + NAD(P)H

N,N-dimethylformamide + H(2)O = dimethylamine + formate

(1a) ATP + [DNA ligase]-L-lysine = 5'-adenosyl [DNA ligase]-N(epsilon)-phosphono-L-lysine + diphosphate

L-histidinol + H(2)O + 2 NAD(+) = L-histidine + 2 NADH

UDP-glucose + 2 NAD(+) + H(2)O = UDP-glucuronate + 2 NADH

Acetyl-CoA + maltose = CoA + acetyl-maltose

GTP + H(2)O = GDP + phosphate

Myo-inositol hexakisphosphate + H(2)O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate

ATP + pyruvate = ADP + phosphoenolpyruvate

10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe)

(R)-pantoate + NADP(+) = 2-dehydropantoate + NADPH

Anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD(+) = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 NADH

Formamide + H(2)O = formate + NH(3)

Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)

Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.

ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate

2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O

L-proline + a quinone = (S)-1-pyrroline-5-carboxylate + a quinol

Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.

3-methyl-2-oxobutanoate + [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] lipoyllysine = [dihydrolipoyllysine-residue (2-methylpropanoyl)transferase] S-(2-methylpropanoyl)dihydrolipoyllysine + CO(2)

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides

S-adenosyl-L-methionine + 3,4-dihydroxy-trans-cinnamate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-trans-cinnamate

ATP + thiamine = AMP + thiamine diphosphate

UDP-alpha-D-glucose = UDP-alpha-D-galactose

Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose

H(2) + 2 ferricytochrome c3 = 2 H(+) + 2 ferrocytochrome c3

ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine

2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate

Hydrolysis of terminal non-reducing alpha-L-rhamnose residues in alpha-L-rhamnosides

(S)-3-hydroxybutanoyl-CoA + NADP(+) = 3-acetoacetyl-CoA + NADPH

ATP + (R)-5-diphosphomevalonate = ADP + phosphate + isopentenyl diphosphate + CO(2)

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids

ATP + D-ribose = ADP + D-ribose 5-phosphate

[Molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP + [cysteine desulfurase]-S-sulfanyl-L-cysteine + reduced acceptor = AMP + [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + [cysteine desulfurase] + oxidized acceptor

S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-methylthio-2-oxobutanoate + 7,8-diaminononanoate

D-ribose 5-phosphate = D-ribulose 5-phosphate

(1a) dTDP-beta-L-evernosamine + NADPH + O(2) = dTDP-N-hydroxy-beta-L-evernosamine + NADP(+) + H(2)O

L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate + NH(3) + NADPH

Random hydrolysis of (1->6)-alpha-D-mannosidic linkages in unbranched (1->6)-mannans

A C(21)-steroid + [reduced NADPH--hemoprotein reductase] + O(2) = a 17-alpha-hydroxy-C(21)-steroid + [oxidized NADPH--hemoprotein reductase] + H(2)O

Alpha-D-glucose = beta-D-glucose

Beta-nicotinate D-ribonucleotide + diphosphate + CO(2) = pyridine-2,3-dicarboxylate + 5-phospho-alpha-D-ribose 1-diphosphate

ATP + N-acyl-D-mannosamine = ADP + N-acyl-D-mannosamine 6-phosphate

ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate

Similar to that of papain.

D-glucose + NAD(P)(+) = D-glucono-1,5-lactone + NAD(P)H

Acetyl-CoA + 3-methyl-2-oxobutanoate + H(2)O = (2S)-2-isopropylmalate + CoA

4-amino-5-aminomethyl-2-methylpyrimidine + H(2)O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + ammonia

(R)-mevalonate + CoA + 2 NADP(+) = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH

ATP + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly = diphosphate + [molybdopterin-synthase sulfur-carrier protein]-Gly-Gly-AMP

UDP-N-acetyl-alpha-D-glucosamine + H(2)O = N-acetyl-D-mannosamine + UDP

H(2) + oxidized coenzyme F420 = reduced coenzyme F420

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products

N-acetyl-alpha-D-glucosamine 1-phosphate = N-acetyl-D-glucosamine 6-phosphate

Chorismate = prephenate

Nitrous oxide + 2 ferricytochrome c + H(2)O = 2 nitric oxide + 2 ferrocytochrome c + 2 H(+)

Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides

2 pyruvate = 2-acetolactate + CO(2)

IMP + H(2)O = inosine + phosphate

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO(2)

17-alpha-hydroxypregnenolone + [reduced NADPH--hemoprotein reductase] + O(2) = 3-beta-hydroxyandrost-5-en-17-one + acetate + [oxidized NADPH--hemoprotein reductase] + H(2)O

D-allothreonine = glycine + acetaldehyde

((1->2)-beta-D-glucosyl)(n) + H(2)O = sophorose + ((1->2)-beta-D-glucosyl)(n-2)

7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde

Hydrolysis of Xaa-|-Pro dipeptides. Also acts on aminoacyl-hydroxyproline analogs. No action on Pro-|-Pro.

Choline = trimethylamine + acetaldehyde

4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole + HCO(3)(-) = ADP + phosphate + 5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole

(S)-lactaldehyde + NAD(+) + H(2)O = (S)-lactate + NADH

Xylitol + NADP(+) = L-xylulose + NADPH

L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H

D-glucose 6-phosphate = D-fructose 6-phosphate

ATP + L-phenylalanine + H(2)O = AMP + diphosphate + D-phenylalanine

ATP + 2-succinylbenzoate + CoA = AMP + diphosphate + 2-succinylbenzoyl-CoA

1D-chiro-inositol + NAD(+) = 2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH

Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate

2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol

Succinate semialdehyde + NADP(+) + H(2)O = succinate + NADPH

A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O

S-adenosyl-L-methionine + ethanolamine phosphate = S-adenosyl-L-homocysteine + N-methylethanolamine phosphate

Alpha-maltose 1-phosphate + ((1->4)-alpha-D-glucosyl)(n) = phosphate + ((1->4)-alpha-D-glucosyl)(n+2)

GlcNAc-MurNAc-L-alanyl-gamma-D-glutamyl-meso-diaminopimelyl-D-alanine + H(2)O = GlcNAc-MurNAc-L-alanyl-gamma-D-glutamyl-meso-diaminopimelate + D-alanine

Phosphoenolpyruvate + D-arabinose 5-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate 8-phosphate + phosphate

Glycolate + NADP(+) = glyoxylate + NADPH

D-glycerate + NAD(P)(+) = hydroxypyruvate + NAD(P)H

Hydrolysis of an N(6)-acetyl-lysine residue of a histone to yield a deacetylated histone

ATP + phenylacetate + CoA = AMP + diphosphate + phenylacetyl-CoA

Shikimate + NADP(+) = 3-dehydroshikimate + NADPH

D-gluconate + NAD(P)(+) = 5-dehydro-D-gluconate + NAD(P)H

(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate

Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose

ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate

Acetyl-CoA + n malonyl-CoA + 2n NADPH = long-chain-acyl-CoA + n CoA + n CO(2) + 2n NADP(+)

Acetyl-CoA + [acyl-carrier-protein] = CoA + acetyl-[acyl-carrier-protein]

Myo-inositol + NAD(+) = 2,4,6/3,5-pentahydroxycyclohexanone + NADH

(S)-ureidoglycolate = glyoxylate + urea

Linoleate + O(2) = (9Z,12Z)-(11S)-11-hydroperoxyoctadeca-9,12-dienoate

Propane-1,2-diol = propanal + H(2)O

L-arginine + H(2)O = L-ornithine + urea

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA

L-erythrulose 1-phosphate = D-erythrulose 4-phosphate

Eliminative cleavage of alginate to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enuronosyl groups at their non-reducing ends and beta-D-mannuronate at their reducing end.

(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH

2-deoxy-D-ribosyl-base(1) + base(2) = 2-deoxy-D-ribosyl-base(2) + base(1) 

L-rhamnonate = 2-dehydro-3-deoxy-L-rhamnonate + H(2)O

Meso-2,6-diaminoheptanedioate = L-lysine + CO(2)

Isocitrate = succinate + glyoxylate

5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate

5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH

Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro.

An alpha-L-fucoside + H(2)O = L-fucose + an alcohol

Glycerol = 3-hydroxypropanal + H(2)O

2 glutathione + ROOH = glutathione disulfide + H(2)O + ROH

A 2'-deoxyribonucleoside 5'-monophosphate + H(2)O = a 2'-deoxyribonucleoside + phosphate

(S)-malate = fumarate + H(2)O

Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine

Chitin + H(2)O = chitosan + acetate

Hg + NADP(+) + H(+) = Hg(2+) + NADPH

S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA 

S-(5-deoxy-D-ribos-5-yl)-L-homocysteine = L-homocysteine + (4S)-4,5-dihydroxypentan-2,3-dione

Myo-inositol phosphate + H(2)O = myo-inositol + phosphate

Urate + O(2) + H(2)O = 5-hydroxyisourate + H(2)O(2)

S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA 

5-hydroxyisourate + H(2)O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate

Phenylacetaldehyde + NAD(+) + H(2)O = phenylacetate + NADH

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate

Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans

8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate

Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-

Beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate

D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH

UDP-N-acetyl-alpha-D-mannosamine + N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol = UDP + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol

D-glucono-1,5-lactone + H(2)O = D-gluconate

2-C-methyl-D-erythritol 4-phosphate + NADP(+) = 1-deoxy-D-xylulose 5-phosphate + NADPH

Phosphoenolpyruvate + D-erythrose 4-phosphate + H(2)O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate

(R)-mevalonate + CoA + 2 NAD(+) = 3-hydroxy-3-methylglutaryl-CoA + 2 NADH

dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose

(S)-malate + NAD(+) = oxaloacetate + NADH

GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H(2)O

D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H(2)O

(1a) a nucleoside 5'-triphosphate + H(2)O = a nucleoside 5'-diphosphate + phosphate

6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H(2)O = D-glucose + D-glucose 6-phosphate

Taurine + 2-oxoglutarate + O(2) = sulfite + aminoacetaldehyde + succinate + CO(2)

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate

5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate

Hydrolysis of terminal non-reducing beta-D-fructofuranoside residues in beta-D-fructofuranosides

Glutarate + 2-oxoglutarate + O(2) = (S)-2-hydroxyglutarate + succinate + CO(2)

3-beta-D-glucosyl-D-glucose + phosphate = D-glucose + alpha-D-glucose 1-phosphate

Hydrolysis of the 1,3-alpha-L-galactosidic linkages of neoagaro-oligosaccharides that are smaller than a hexamer, yielding 3,6-anhydro-L-galactose and D-galactose

L-erythro-3,5-diaminohexanoate + H(2)O + NAD(+) = (S)-5-amino-3-oxohexanoate + NH(3) + NADH

ATP + L-fuculose = ADP + L-fuculose 1-phosphate

ATP + H(2)O + vitamin B12-[cobalamin-binding protein](Side 1) = ADP + phosphate + vitamin B12(Side 2) + [cobalamin-binding protein](Side 1)

L-threonine = glycine + acetaldehyde

D-firefly luciferin + O(2) + ATP = firefly oxyluciferin + CO(2) + AMP + diphosphate + light

L-glutamate + H(2)O + NAD(+) = 2-oxoglutarate + NH(3) + NADH

L-ornithine + a 2-oxo acid = L-glutamate 5-semialdehyde + an L-amino acid

P(1),P(4)-bis(5'-guanosyl) tetraphosphate + H(2)O = GTP + GMP

(1a) L-methionine = methanethiol + 2-aminobut-2-enoate

L-histidinol phosphate + H(2)O = L-histidinol + phosphate

Chorismate = isochorismate

Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA

UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose

(9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate = (15Z)-12-oxophyto-10,15-dienoate

Phosphonoacetaldehyde + H(2)O = acetaldehyde + phosphate

1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate

(2R,3S)-2-methylmalyl-CoA = propanoyl-CoA + glyoxylate

2'-deoxycytidine + H(2)O = 2'-deoxyuridine + NH(3)

Acetyl-CoA + phosphate = CoA + acetyl phosphate

Nicotinamide + H(2)O = nicotinate + NH(3)

A 5'-phosphopolynucleotide + H(2)O = a polynucleotide + phosphate

(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate = 8 diphosphate + di-trans,octa-cis-undecaprenyl diphosphate

Acetyl-CoA + L-homoserine = CoA + O-acetyl-L-homoserine

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn)

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides

ADP-D-ribose 1''-phosphate + H(2)O = ADP-D-ribose + phosphate

6-phospho-D-glucono-1,5-lactone + H(2)O = 6-phospho-D-gluconate

A 4-O-methyl-D-glucopyranuronate ester + H(2)O = 4-O-methyl-D-glucuronic acid + an alcohol

CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]

L-methionine + thioredoxin disulfide + H(2)O = L-methionine (S)-S-oxide + thioredoxin

ATP + H(2)O + cellular protein(Side 1) = ADP + phosphate + cellular protein(Side 2)

DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine

ATP + D-xylulose = ADP + D-xylulose 5-phosphate

(1a) 1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate

7,8-dihydroneopterin 3'-triphosphate + H(2)O = 6-carboxy-5,6,7,8-tetrahydropterin + acetaldehyde + triphosphate

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate

N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate

ATP + H(2)O + H(+)(Side 1) = ADP + phosphate + H(+)(Side 2)

Hydrolysis of (1->4)-linkages in (1->4)-beta-D-glucans, to remove successive glucose units

L-lysine = cadaverine + CO(2)

Alpha-D-glucosamine 6-phosphate + H(2)O = D-fructose 6-phosphate + NH(3)

Formyl-CoA + oxalate = formate + oxalyl-CoA

7-alpha,12-alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA = 12-alpha-hydroxy-3-oxochola-4,6-dien-24-oyl-CoA + H(2)O

N(6)-(L-1,3-dicarboxypropyl)-L-lysine + NAD(+) + H(2)O = L-lysine + 2-oxoglutarate + NADH

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione

(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O

4-(2-carboxyphenyl)-4-oxobutanoyl-CoA = 1,4-dihydroxy-2-naphthoyl-CoA + H(2)O

Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.

ATP + 3'-dephospho-CoA = ADP + CoA

ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole

Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO(2)

7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

Oxalyl-CoA = formyl-CoA + CO(2)

L-fuculose 1-phosphate = glycerone phosphate + (S)-lactaldehyde

CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol

(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m)

(S)-dihydroorotate + fumarate = orotate + succinate

2-alpha-D-glucosyl-D-glucose + phosphate = D-glucose + beta-D-glucose 1-phosphate

An aldehyde + H(2)O + 2 oxidized ferredoxin = a carboxylate + 2 H(+) + 2 reduced ferredoxin

S-(hydroxymethyl)glutathione + NAD(P)(+) = S-formylglutathione + NAD(P)H

L-alanine + H(2)O + NAD(+) = pyruvate + NH(3) + NADH

2'-(5-triphosphoribosyl)-3'-dephospho-CoA + citrate lyase apo-[acyl-carrier protein] = citrate lyase holo-[acyl-carrier protein] + diphosphate

RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O

A monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)

(S)-2-haloacid + H(2)O = (R)-2-hydroxyacid + halide

O-phospho-L(or D)-serine + H(2)O = L(or D)-serine + phosphate

Hydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides so as to remove successive maltose units from the non-reducing ends of the chains

5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(In) = 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na(+)(Out)

Xyloglucan + H(2)O = xyloglucan oligosaccharides

Sphingomyelin + H(2)O = N-acylsphingosine + phosphocholine

Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate

D-fructose 6-phosphate + phosphate = acetyl phosphate + D-erythrose 4-phosphate + H(2)O

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.

2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP

A short-chain (3S)-3-hydroxyacyl-CoA = a short-chain trans-2-enoyl-CoA + H(2)O

ATP + L-aspartate = ADP + 4-phospho-L-aspartate

ATP + thiamine phosphate = ADP + thiamine diphosphate

Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine

S-adenosyl-L-methionine + a 5'-(5'-triphosphoguanosine)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-[mRNA]

L-aspartate = fumarate + NH(3)

Preferential cleavage with hydrophobic residues at P2, P1 and P1'.

Acyl-CoA + NAD(+) = trans-didehydroacyl-CoA + NADH

Endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products

R-CHOH-R' + NADP(+) = R-CO-R' + NADPH

(1a) L-serine = 2-aminoprop-2-enoate + H(2)O

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate

GTP + a 5'-diphospho-[mRNA] = diphosphate + a 5'-(5'-triphosphoguanosine)-[mRNA]

Glycerol + NAD(+) = glycerone + NADH

(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD(+) = 2-methylacetoacetyl-CoA + NADH

NAD(H) + H(2)O = AMP + NMN(H)

H(2) + 5,10-methenyltetrahydromethanopterin = H(+) + 5,10-methylenetetrahydromethanopterin

4-coumarate = 4-vinylphenol + CO(2)

An aryl sulfate + a phenol = a phenol + an aryl sulfate

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)

An acylphosphate + H(2)O = a carboxylate + phosphate

Uroporphyrinogen III = coproporphyrinogen + 4 CO(2)

S-adenosyl-L-methionine + cytidine(32) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(32) in tRNA

GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol = GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol

Cyclomaltodextrin + H(2)O = linear maltodextrin

Hydrolysis of the -Gly-|-Gly- bond in the pentaglycine inter-peptide link joining staphylococcal cell wall peptidoglycans.

L-histidine-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-((3S)-3-amino-3-carboxypropyl)-L-histidine-[translation elongation factor 2] + S-methyl-5'-thioadenosine

ATP + acetate + CoA = ADP + phosphate + acetyl-CoA

ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate

Pyruvate + phosphate + O(2) = acetyl phosphate + CO(2) + H(2)O(2)

An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH

Random hydrolysis of (1->2)-glucosidic linkages in (1->2)-beta-D-glucans

10-formyltetrahydrofolate + NADP(+) + H(2)O = tetrahydrofolate + CO(2) + NADPH

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA

ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]

ATP + riboflavin = ADP + FMN

Protein dithiol + NAD(P)(+) = protein disulfide + NAD(P)H

A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid

Carbamoyl phosphate + N(2)-acetyl-L-ornithine = phosphate + N-acetyl-L-citrulline

(R)-lactate + N-acetyl-D-glucosamine 6-phosphate = N-acetylmuramate 6-phosphate + H(2)O

Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO(2) 

ATP + FMN = diphosphate + FAD

Acetyl-CoA + n malonyl-CoA + 2n NADPH = a long-chain fatty acid + (n+1) CoA + n CO(2) + 2n NADP(+)

5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP(+) = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NADPH

L-lysine + O(2) = 5-aminopentanamide + CO(2) + H(2)O

UDP-N-acetyl-alpha-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1)

A pyrimidine nucleoside + H(2)O = D-ribose + a pyrimidine base

2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + H(2)O = 5-amino-6-(5-phosphoribosylamino)uracil + NH(3)

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine

Preferential cleavage of bonds with hydrophobic residues in P1'.

Protein L-glutamine + H(2)O = protein L-glutamate + NH(3)

ATP + L-serine + tRNA(Sec) = AMP + diphosphate + L-seryl-tRNA(Sec)

ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)

Penicillin + H(2)O = a carboxylate + 6-aminopenicillanate

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)

A protein-L-glutamine + a protein-L-lysine = a protein with an N(6)-(gamma-glutamyl)-L-lysine cross-link + NH(3)

A long-chain aldehyde + O(2) + 2 NADPH = an alkane + formate + H(2)O + 2 NADP(+)