› 3bbk

X-ray diffraction
2.75Å resolution

Miminally Junctioned Hairpin Ribozyme Incorporates A38C and 2'5'-phosphodiester Linkage within Active Site

Released:
DOI: 10.2210/pdb3bbk/pdb
PDB 3bbk coloured by chain and viewed from the front.
PDB 3bbk coloured by chain and viewed from the side.
PDB 3bbk coloured by chain and viewed from the top.
Source organism: Synthetic construct
Primary publication:
Structural effects of nucleobase variations at key active site residue Ade38 in the hairpin ribozyme.
MacElrevey C, Salter JD, Krucinska J, Wedekind JE
RNA 14 1600-16 (2008)
PMID: 18596253

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct RNA molecules
Macromolecules (3 distinct):
Loop A Substrate strand Chain: A
Molecule details ›
Chain: A
Length: 13 nucleotides
Theoretical weight: 4.05 KDa
Source organism: Synthetic construct
Expression system: Not provided
Loop A and Loop B Ribozyme strand Chain: B
Molecule details ›
Chain: B
Length: 30 nucleotides
Theoretical weight: 9.76 KDa
Source organism: Synthetic construct
Expression system: Not provided
Loop B S-turn strand Chain: C
Molecule details ›
Chain: C
Length: 19 nucleotides
Theoretical weight: 5.97 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand NCO 3 x NCO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P6122
Unit cell:
a: 93.52Å b: 93.52Å c: 133.97Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.197 0.195 0.221
Expression system: Not provided

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Citations

4 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
3 more