3bbd

X-ray diffraction
2.15Å resolution

M. jannaschii Nep1 complexed with S-adenosyl-homocysteine

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal RNA small subunit methyltransferase Nep1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 205 amino acids
Theoretical weight: 24.45 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57977 (Residues: 1-205; Coverage: 100%)
Gene names: MJ0557, nep1
Sequence domains: EMG1/NEP1 methyltransferase
Structure domains: Alpha/beta knot

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 84.894Å b: 91.121Å c: 93.713Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.18 0.221
Expression system: Escherichia coli BL21(DE3)