X-ray diffraction
2.3Å resolution

Crystal structure of the E. coli pyrimidine nucleoside hydrolase YeiK in complex with inosine


Function and Biology Details

Reaction catalysed:
A pyrimidine nucleoside + H(2)O = D-ribose + a pyrimidine base
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Pyrimidine-specific ribonucleoside hydrolase RihB Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 333 amino acids
Theoretical weight: 35.93 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P33022 (Residues: 1-313; Coverage: 100%)
Gene names: JW2149, b2162, rihB, yeiK
Sequence domains: Inosine-uridine preferring nucleoside hydrolase
Structure domains: Ribonucleoside hydrolase-like

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: P1
Unit cell:
a: 45.112Å b: 82.406Å c: 90.163Å
α: 67.9° β: 79.59° γ: 89.65°
R R work R free
0.203 0.201 0.255
Expression system: Escherichia coli BL21(DE3)