› 3b9t

X-ray diffraction
1.58Å resolution

Crystal structure of predicted acetamidase/formamidase (YP_546212.1) from Methylobacillus flagellatus KT at 1.58 A resolution

Released:
DOI: 10.2210/pdb3b9t/pdb
PDB entry 3b9t coloured by chain, front view.
PDB entry 3b9t coloured by chain, side view.
PDB entry 3b9t coloured by chain, top view.
Source organism: Methylobacillus flagellatus KT
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-172857 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Twin-arginine translocation pathway signal Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 484 amino acids
Theoretical weight: 53.56 KDa
Source organism: Methylobacillus flagellatus KT
Expression system: Escherichia coli
UniProt:
  • Canonical: Q1GZG6 (Residues: 1-483; Coverage: 100%)
Gene name: Mfla_2104
Sequence domains: Acetamidase/Formamidase family

Ligands and Environments

3 bound ligands:
Ligand MG 8 x MG
Ligand CL 2 x CL
Ligand EDO 41 x EDO
1 modified residue:
Ligand MSE 52 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P1
Unit cell:
a: 62.165Å b: 82.723Å c: 83.576Å
α: 107.82° β: 105.81° γ: 95.18°
R-values:
R R work R free
0.14 0.138 0.177
Expression system: Escherichia coli

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