X-ray diffraction
2.08Å resolution

Crystal structure of zebrafish prostacyclin synthase (cytochrome P450 8A1)


Function and Biology Details

Reactions catalysed:
A hydroperoxy icosatetraenoate = an oxoicosatetraenoate + H(2)O
(5Z,13E)-(15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-6,9-alpha-epoxy-11-alpha,15-dihydroxyprosta-5,13-dienoate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Prostacyclin synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 475 amino acids
Theoretical weight: 54.85 KDa
Source organism: Danio rerio
Expression system: Escherichia coli BL21(DE3)
  • Canonical: F1RE08 (Residues: 17-480; Coverage: 97%)
Gene names: ptgis, ptgisl
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

Cofactor: Ligand HEM 2 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 58.667Å b: 87.896Å c: 190.854Å
α: 90° β: 90° γ: 90°
R R work R free
0.229 0.227 0.264
Expression system: Escherichia coli BL21(DE3)