› 3b7n

X-ray diffraction
1.86Å resolution

Crystal Structure of Yeast Sec14 Homolog Sfh1 in Complex with Phosphatidylinositol

Released:
DOI: 10.2210/pdb3b7n/pdb
PDB entry 3b7n coloured by chain, front view.
PDB entry 3b7n coloured by chain, side view.
PDB entry 3b7n coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Functional anatomy of phospholipid binding and regulation of phosphoinositide homeostasis by proteins of the sec14 superfamily.
Schaaf G, Ortlund EA, Tyeryar KR, Mousley CJ, Ile KE, Garrett TA, Ren J, Woolls MJ, Raetz CR, Redinbo MR, Bankaitis VA
Mol Cell 29 191-206 (2008)
PMID: 18243114

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152589 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRAL-TRIO domain-containing protein YKL091C Chain: A
Molecule details ›
Chain: A
Length: 320 amino acids
Theoretical weight: 37.41 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P33324 (Residues: 1-310; Coverage: 100%)
Gene name: YKL091C
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand PO4 2 x PO4
Ligand ACT 1 x ACT
Ligand B7N 1 x B7N
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 50.158Å b: 71.407Å c: 91.177Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.233
Expression system: Escherichia coli

Quick links

Citations

46 review citations

Phosphoinositides: tiny lipids with giant impact on cell regulation.
Balla T. (2013)
45 more

21 mentions without citation

The Sec14 superfamily and mechanisms for crosstalk between lipid metabolism and lipid signaling.
Bankaitis et al. (2010)
20 more